Product Introduction
Reactive oxygen species (ROS) are a class of highly reactive oxygen species, including oxygen anions (O2- ), hydrogen peroxide (H2O2),hydroxyl radicals (OH- ), and nitric oxide , etc. Reactive oxygen species play a complex role in organisms, and are closely related to the regulation of normal physiological functions and the occurrence and development of diseases.
Product Features
Compared with DCFH-DA used in the regular version of Reactive Oxygen Species (ROS) Assay Kit(BC00009), the product of H2DCF-DA after hydrolysis and oxidation has an additional negative charge, which hinders its infiltration out of cells, thus having lower background and better fluorescence signal.
Principle
The enhanced reactive oxygen species assay kit is a kit that uses the fluorescent probe H2DCF-DA to detect reactive oxygen species. The probe H2DCF-DA itself has no fluorescence and can freely pass through the cell membrane. After entering the cell, it can be hydrolyzed by esterases in the cell and oxidized by ROS, and converted into a green fluorescent form. The fluorescence of DCF can be used to determine the level of reactive oxygen species in the cell. This kit provides the reactive oxygen species positive control reagent Rosup to facilitate the detection of reactive oxygen species.
Components
Serial number | Components | Size (100T) | Storage |
Reagent 1 | H2DCF-DA (10mM) | 0.1 ml | -20℃, avoid light , store at 4℃ after opening, valid for 6 months. |
Reagent 2 | Reactive oxygen positive control (Rosup, 50mg/ml) | 1 ml | -20℃, avoid light , store at 4℃ after opening, valid for 6 months. |
Storage
Unopened kit can be stored at -20°C for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
