Product Introduction
Reactive oxygen species (ROS) are a class of highly reactive oxygen species, including oxygen anions (O2- ), hydrogen peroxide (H2O2 ), hydroxyl radicals (OH- ), and nitric oxide , etc. Reactive oxygen species play a complex role in organisms, and are closely related to the regulation of normal physiological functions and the occurrence and development of diseases.
Product Features
Low background, high sensitivity, wide linear range, and easy to use.
Principle
Reactive Oxygen Species (ROS) Assay Kit is a kit that uses the fluorescent probe DCFH-DA to detect reactive oxygen species. DA itself has no fluorescence and can freely pass through the cell membrane. After entering the cell, it can be hydrolyzed by the esterase in the cell to produce DCFH. However, DCFH cannot penetrate the cell membrane, making it easy for the probe to be loaded into the cell. The reactive oxygen species in the cell can oxidize the non-fluorescent DCFH to produce fluorescent DCF. By detecting the fluorescence of DCF, the level of reactive oxygen species in the cell can be known.
Components
For cells cultured in 6-well plates, this kit can perform 100-500 tests.
Serial number | Components | Size (100T-500T) | Storage |
Reagent 1 | DCFH-DA (10mM) | 0.1ml | -20℃, avoid light , store at 4℃ after opening, valid for 6 months. |
Reagent 2 | Reactive Oxygen Species Positive Control (Rosup, 50mg/ml) | 1ml | -20℃, avoid light , store at 4℃ after opening, valid for 6 months. |
Storage
Unopened kit can be stored at -20°C for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
