Product Introduction
The Fluorescein 647 -Annexin V/PI Apoptosis Detection Kit provides a rapid and simple method to Detect apoptosis by labeling early apoptotic cells (Far-Red) and necrotic or late apoptotic cells (red). Annexin V is a Ca²⁺-dependent phospholipid-binding protein with a molecular weight of 35–36 kDa that selectively binds to phosphatidylserine (PS). In normal cells, PS is located on the inner side of the plasma membrane. During early apoptosis, PS is translocated to the outer surface of the cell membrane and exposed to the extracellular environment. By binding Fluorescein 647 -labeled Annexin V to the exposed PS, this hallmark of apoptosis can be directly detected using flow cytometry or fluorescence microscopy.
Propidium Iodide (PI) is a DNA-binding dye that stains the nuclei of necrotic or late apoptotic cells that have lost membrane integrity, emitting red fluorescence upon excitation.
Basic Information
Product name | Fluorescein 647-Annexin V/PI Apoptosis Kits( Far-Red /Red) |
Sizes | 20 T/50 T/100 T |
Storage | 2-8℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Ex/Em | Fluorescein 647-Annexin V：650/668 nm ；PI：535/617 nm |
Product Components
Components | 10 T | 50 T | 100 T |
A.1 × Annexin V Binding Buffer | 10 mL | 50 mL | 2 × 50 mL |
B.Fluorescein 647-Annexin V | 50 μL | 250 μL | 500 μL |
C.PI | 100 μL | 500 μL | 1 mL |
Note: Do not freeze Component B.
Notes
1. Briefly centrifuge reagents before use.
2. To slow apoptosis, incubate on ice, but extend time to ≥30 min.
3. Analyze samples within 1 h post-staining.
4. Use a large pipette tip with a small tip attached to avoid cell loss.
5. Protect dyes from light to prevent quenching.
6. For research use only. Not for diagnostic or therapeutic use.
7. Wear lab coat and gloves for safety.
This product is for research use only！
