Product Introduction
Carbonic anhydrase (CA) is a metalloenzyme with Zn 2+ as the active center. It can be used to efficiently catalyze the reversible hydration reaction of CO 2: CO 2 +H 2 O ⇋ HCO 3 - +H + . The catalytic rate can reach 107 times that under natural conditions, making it one of the fastest enzymes known to date.
Detection principle
Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to produce light yellow p-nitrophenol. The activity of carbonic anhydrase can be reflected by detecting the rate of increase of absorbance at 405 nm.
The following standard curve is for reference only:
Product Composition
Serial Number | Product Name | Packing Specifications (100T) | Storage |
Reagent 1 | Extract | 15 mL | -20℃, can be stored at room temperature after opening. |
Reagent 2 | Enzyme solution | 1mL/tube, 2 tubes | -20℃, store at 4°C after opening. |
Reagent 3 | Substrate working solution | 3 mL | -20℃ |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage Conditions
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, shall not be used as food or medicine, and shall not be stored in ordinary residences.
