Product Introduction
Carbonic anhydrase (CA) is a metalloenzyme with Zn 2+ as the active center. It can be used to efficiently catalyze the reversible hydration reaction of CO 2: CO 2 +H 2 O ⇋ HCO 3 - +H + . The catalytic rate can reach 107 times that under natural conditions, making it one of the fastest enzymes known to date.
Detection principle
Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to produce light yellow p-nitrophenol. The activity of carbonic anhydrase can be reflected by detecting the rate of increase of absorbance at 405 nm.
The following standard curve is for reference only:
Product Composition
Serial Number | Product Name | Packing Specifications (100T) | Storage |
Reagent 1 | Extract | 15 mL | -20℃, can be stored at room temperature after opening. |
Reagent 2 | Enzyme solution | 1mL/tube, 2 tubes | -20℃, store at 4°C after opening. |
Reagent 3 | Substrate working solution | 3 mL | -20℃ |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage Conditions
The unopened kit can be stored at -20℃ for 12 months.
