Product Introduction
Total Bilirubin (TBIL) is the sum of bilirubin in the blood, including unconjugated bilirubin (indirect bilirubin) and conjugated bilirubin (direct bilirubin). Bilirubin is a yellow substance produced after the aging and decomposition of red blood cells. It is metabolized in the liver and then excreted from the body in the form of bile. The determination of total bilirubin level is an important indicator for evaluating liver function, biliary system, and red blood cell production and destruction .
Features
Easy to operate, the test can be completed within 5 minutes .
Detection Principle
Direct bilirubin in serum can react directly with the diazo reagent to generate azo bilirubin in an acidic environment. Under the action of the accelerator, the intramolecular hydrogen bond of unconjugated bilirubin is destroyed and it reacts with the diazo reagent to generate azo bilirubin. The absorbance of the complex obtained by this reaction at 570 nm is proportional to the total bilirubin concentration in the sample.
Total bilirubin determined by this kit. The following standard curve is for reference only:
Product composition
Serial Number | Product Name | Packing Specifications ( 100T ) | Storage |
Reagent 1 | R1 | 30mL | Store at -20℃ away from light , store at 2-8℃ after opening |
Reagent 2 | R2 | 15mL | Store at -20℃ away from light , store at 2-8℃ after opening |
Reagent 3 | R3 | 3mL | Store at -20℃ , store at 2-8 ℃ after opening |
Reagent 4 | Standard（50mM） | 1mL | Store at -20℃ , store at 2-8 ℃ after opening |
Consumable 1 | 96-well ELISA plate | 1 plate | RT |
Consumable 2 | 96-well membrane | 2 pieces | RT |
Storage conditions
The unopened kit can be stored at -20°C for 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.
