Product Introduction
Alanine aminotransferase, also known as alanine aminotransferase, is mainly distributed in animal livers, but also exists in plants, microorganisms and cultured cells, and is essential for amino acid metabolism. In the diagnosis of liver diseases, liver cell damage can cause alanine aminotransferase to be released from the cells into the blood, thereby causing an increase in serum alanine aminotransferase activity. Therefore, alanine aminotransferase is a sensitive and commonly used indicator for assessing liver function damage.
Detection Principle
Alanine aminotransferase (ALT) acts on substrates composed of alanine and α-ketoglutarate at 37°C and pH 7.4 to generate pyruvate and glutamate. After 30 min of reaction (fixed time), 2,4-dinitrophenylhydrazine (DNPH) hydrochloric acid solution is added to terminate the reaction. At the same time, DNPH adds to the carbonyl group in ketoacid to generate pyruvate phenylhydrazone. Phenylhydrazone is reddish brown under alkaline conditions. The absorbance is measured at 510 nm and the enzyme activity is calculated.
Product Composition
Serial Number | Product Name | Packing Specifications ( 100T ) | Storage |
Reagent 1 | Substrate matrix liquid | 5 mL | 2-8 ℃ after opening . |
Reagent 2 | Color developer | 5 mL | 2-8 ℃ away from light after opening . |
Reagent 3 | Alkaline solution | 5 mL | 2-8 ℃ after opening . |
Reagent 4
| 2 μmol /mL Sodium Pyruvate Standard | 0.5 mL | 2-8 ℃ after opening . |
Reagent 5 | Buffer | 0.5 mL | 2-8 ℃ after opening . |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage conditions
The unopened test kit can be stored at -20 ℃ for 12 months, and after opening, it can be stored at 2-8 ℃ for 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.
