Product Introduction
Urea is the main end product of protein metabolism in the human body, and it constitutes the vast majority of non-protein nitrogen in the blood. Urea nitrogen in the blood originates from the liver and is excreted from the body through the kidneys with urine. Kidney failure, nephritis, urinary tract obstruction, etc. can increase the blood urea nitrogen content.
Principle
Urea is hydrolyzed by urease to produce ammonia ions and carbon dioxide. The ammonia ions react with the phenol color developer in an alkaline medium to produce a blue-green substance. The figure below shows the standard curve of urea determination by this kit.
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | 100mmol/L Urea Standard | 200 μL | -20℃, store at 2-8°C after opening. |
Reagent 2 | Enzyme Diluent | 7.5 mL | -20℃, store at 2-8°C after opening. |
Reagent 3 | Enzyme Solution | 25 μL | -20℃, protect from light, store at 2-8°C after opening. |
Reagent 4 | Phenol Color Developer | 15 mL | -20℃, protect from light, store at 2-8°C after opening. |
Reagent 5 | Alkaline Sodium Hypochlorite Solution | 15 mL | -20℃, protect from light, store at 2-8°C after opening. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months, and after opening, it can be stored at 2-8℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
