Product Introduction
Protein content determination is one of the most commonly used and basic analytical methods in biochemical research.
Product Features
This kit can rapidly measure protein in trace amounts. It is easy to operate, fast and time-saving, can save a lot of reagents, and can measure a large number of samples at one time.
Principle
Any compound containing two carbamoyl groups (-CONH2) in the molecule can react with alkaline copper solution to form a purple complex. This reaction is called biuret reaction. There are many peptide bonds (-CONH-) in protein molecules that can react in this way. The color development degree of various proteins is basically the same. The absorbance can be detected at 520-580nm (the best detection wavelength is 540nm). The darker the purple color of the product, the higher the protein content, and vice versa. Based on this, the protein content can be calculated through colorimetric analysis.
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Copper Sulfate | powder ×1 vial | -20℃, after opening store at 2-8°C for 6 months. |
Reagent 2 | Alkaline Reagent | powder ×1 vial | -20℃, after opening store at 2-8°C in the dark for 6 months. |
Reagent 3 | 100g/L Protein Standard | 575µL ×1 vial | -20℃, after opening store at -20°C for 6 months. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
