DMEM/F12 (with sodium pyruvate, without glucose, glutamine, phenol red)-Cell Medium-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

DMEM/F12 (with sodium pyruvate, without glucose, glutamine, phenol red)

Size1:2L Price1:$20 SKU: CMB0013 Category: Cell Medium Tags: Cell Culture

DMEM/F12 medium is a mixture of DMEM and Ham's F12 medium in a 1:1 ratio. It contains a variety of trace elements and is widely used in the culture of mammalian cells such as MDCK, glial cells, fibroblasts, human endothelial cells and rat fibroblasts.

Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.

L-glutamine is a necessary nutrient element in cell culture, but it is unstable in solution and will degrade spontaneously. The medium without L-glutamine can adjust the amount of L-glutamine according to research needs. Adding fresh L-glutamine or its substitute during use is more conducive to cell growth.

Details

Form	Powder
Size	2L
L-Glutamine	Without
NaHCO3	Without
D-Glucose	Without
Sodium Pyruvate	With
HEPES	Without
Phenol Red	Without
Transportation	Room temperature
Storage	Store at 2-8°C away from light
Shelf Life	24 months