DMEM high glucose (with HEPES, phenol red, glutamine; without sodium pyruvate)

DMEM high glucose (with HEPES, phenol red, glutamine; without sodium pyruvate)

Size1:2L Price1:$20 SKU: CMB0001 Category: Cell Medium Tags:

Datasheet

Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. The glucose content of DMEM medium was originally designed to be 1000 mg/L (low glucose type), and later it was developed to have a glucose content of 4500 mg/L (high glucose type). It is now widely used in the culture of various cells. This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for the culture of various types of cells, but does not contain proteins, lipids or any growth factors, so this product must be used with serum or serum-free additives.

 

HEPES is an excellent biological buffer with no toxic effect on cells. The culture medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.

 

 

 

Details

Form

Powder

Size

2L

L-Glutamine

With

NaHCO3

3.7g/L

D-Glucose

4.5g/L

Sodium Pyruvate

Without

HEPES

With

Phenol Red

With

Transportation

Room temperature

Storage

Store at 2-8°C away from light

Shelf Life

24 months

 

 

Precautions

1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;

2. This product is only used for research or further research, not for diagnosis and treatment.