DMEM high glucose (without HEPES, sodium pyruvate, phenol red, glutamine)-Cell Medium-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. The glucose content of DMEM medium was originally designed to be 1000 mg/L (low glucose type), and later it was developed to have a glucose content of 4500 mg/L (high glucose type). It is now widely used in the culture of various cells. This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for the culture of various types of cells, but does not contain proteins, lipids or any growth factors, so this product must be used with serum or serum-free additives.

Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.

L-glutamine is a necessary nutrient element in cell culture, but it is unstable in solution and will degrade spontaneously. The medium without L-glutamine can adjust the amount of L-glutamine according to research needs. Adding fresh L-glutamine or its substitute during use is more conducive to cell growth.

Details

Form	Powder
Size	2L
L-Glutamine	Without
NaHCO3	With
D-Glucose	4.5g/L
Sodium Pyruvate	Without
HEPES	Without
Phenol Red	Without
Transportation	Room temperature
Storage	Store at 2-8°C away from light
Shelf Life	24 months