Product Introduction
Cell senescence refers to the gradual decline in cell proliferation, differentiation capacity, and physiological functions that occur over time during life activities. Under normal circumstances, the body eliminates senescent cells, while those remaining may accumulate, potentially causing low-level inflammation or further inducing tissue degeneration and carcinogenesis. Key characteristics of cellular senescence include morphological changes, cell cycle arrest, senescence-associated secretory phenotype, macromolecular damage, and metabolic disorders. Currently, the most specific marker for identifying senescence is senescence-associated β-galactosidase (SA-β-Gal), which accumulates with cellular aging and is absent in pre-senescence cells, quiescent cells, and terminally differentiated cells. This kit utilizes the fact that β-Gal in senescent cells can hydrolyze 5-bromo-4-chloro-3-indolyl-β-D-pentose galactoside (X-gal) into a blue substance at pH 6.0, enabling observation of cellular or tissue senescence under optical microscopy.
Scope of Application
Cell aging detection
Product features
Effect is obvious: blue substance is obvious, convenient to observe;
good stability: can withstand 20 times of repeated freeze-thaw and short time normal temperature transportation.
Basic Information
Product name | β-Gal Senescence Staining Kit |
Sizes | 100T |
Storage | -20℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Product Components
components | 100 T |
A. β-Galactoside enzyme staining fixative | 100 mL |
B. β-Galactosidease staining solution A | 1.5 mL |
C. β-Galactosidease staining solution B | 1.5 mL |
D. β-Galactosidease staining solution C | 100 mL |
E. X-Gal solution | 5 mL |
Note: Component E X-Gal solution should be stored in the dark.
Notes
1.Before use, centrifuge the product to the tube bottom immediately before proceeding with subsequent experiments.
2. Remove the bacterial culture medium before staining. Components or free nucleic acids in the medium may be labeled by the dye, causing background interference.
3. The dye should be diluted with 0.85-0.9% physiological saline. Using PBS or HBSS for dilution may affect staining efficacy.
4. Optimize dye concentration and staining time based on specific bacterial samples through condition optimization.
5. For plate-based detection, allow a 10-minute incubation period with residual bacterial liquid for imaging to effectively reduce background noise.
6. Fluorescent dyes are prone to quenching. Ensure proper light protection during operation to minimize quenching effects.
7. For safety, wear lab coats and disposable gloves during handling.
8. This product is strictly for scientific research purposes only and must not be used for clinical diagnosis or treatment.
This product is for research use only！
