Product Introduction
The bacterial viability and toxicity detection kit contains two fluorescent dyes: NucGreen (a green nucleic acid dye that stains both live and dead bacteria) and EthD-III (a red nucleic acid dye that specifically stains dead bacteria with damaged cell membranes). When mixed appropriately, bacteria with intact cell membranes appear green, while those with damaged membranes show distinct green and red fluorescence signals under different detection channels. This kit is suitable for staining most bacterial types and can be analyzed using either fluorescence microscopy or flow cytometry. The excitation and emission spectra of NucGreen and EthD-III are detailed in the product specifications. A common standard for bacterial viability assessment is growth measurement, which evaluates a bacterium's ability to multiply in nutrient-rich culture media. The kit's results demonstrate excellent consistency with growth measurements obtained from both liquid and solid culture media.
Product features
Wide application scope: suitable for most bacteria;
good stability: strong fluorescence brightness and good anti-quenching, stable product, easy to store and transport.
Basic Information
Product name | Viability/Cytotoxicity Assay Kits for Bacteria Cells |
Sizes | 20T/100T |
Storage | -20℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Ex/Em | NucGreen：503/530 nm（combine DNA); EthD-III: 530/620 nm（combine DNA） |
Product Components
components | Specification (20 T) | Specification (100 T) |
A. NucGreen | 20 μL | 100 μL |
B. EthD-III | 40 μL | 200 μL |
Notes
1.Before use, centrifuge the product to the tube bottom immediately before proceeding with subsequent experiments.
2. Remove the bacterial culture medium before staining. Components or free nucleic acids in the medium may be labeled by the dye, causing background interference.
3. The dye should be diluted with 0.85-0.9% physiological saline. Using PBS or HBSS for dilution may affect staining efficacy.
4. Optimize dye concentration and staining time based on specific bacterial samples through condition optimization.
5. For plate-based detection, allow a 10-minute incubation period with residual bacterial liquid for imaging to effectively reduce background noise.
6. Fluorescent dyes are prone to quenching. Ensure proper light protection during operation to minimize quenching effects.
7. For safety, wear lab coats and disposable gloves during handling.
8. This product is strictly for scientific research purposes only and must not be used for clinical diagnosis or treatment.
This product is for research use only！
