Basic information of Human glioblastoma cell line A172
The human glioblastoma cell line A172 is a widely used in vitro model for studying glioblastoma Multiforme (GBM).
1. Source and growth characteristics:
OA172 cells are derived from the brain tumor tissue of a 53 year old male patient and belong to the glioblastoma cell line.
The cell morphology is fibroblast like or epithelioid, grows adherent, and can form clones in semi-solid culture medium, but does not form tumors in immunosuppressed mice.
2. Cultivation conditions:
Recommended culture medium: DMEM high glucose medium (containing 10% fetal bovine serum and 1% penicillin streptomycin solution) [1,4], temperature of 37 ℃, humidity of 70% -80%, gas phase of 95% air and 5% carbon dioxide.The proliferation time is about 26-48 hours, and the doubling time is about 40 hours.
3. Security:
The cell biosafety level is BSL-1, and it does not contain pathogens such as HIV, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
It does not have tumorigenicity in immunosuppressed mice.
4. STR identification:
The oSTR site information includes Amelogenin X, CSF1PO, D13S317, D16S539, etc., ensuring the purity and consistency of the cell line.
5. Biological characteristics
Proliferation and migration: A172 cells have high proliferation and migration abilities, which is consistent with their invasive characteristics in glioblastoma [1,3,8].
Apoptosis and anti apoptosis mechanism: Aspirin induces apoptosis in A172 cells by blocking the IL-6-STAT3 signaling pathway [6]; Resveratrol induces apoptosis by activating Notch-1-dependent p53 to restore glutathione levels [11].
Metabolic characteristics: A172 cells exhibit MTC (mainly oxidative respiration) metabolic characteristics, which may lead to reduced sensitivity to chemotherapy drugs [15].
6. Signal pathways and molecular mechanisms
OTRPM7 and Notch signaling pathway: TRPM7 activates JAK2/STAT3 and Notch signaling pathways in A172 cells, promoting cell proliferation and migration.
OmTOR signaling pathway: The mTOR signaling pathway is activated in A172 cells and may be associated with tumor cell proliferation, survival, and invasion.
OmiR-197 and GAB2: miR-197 promote A172 cell proliferation by upregulating GAB2 expression [9].
OSOX-2 and ROS accumulation: Enhanced expression of SOX-2 is associated with the accumulation of reactive oxygen species (ROS), which may endow A172 cells with dry characteristics [16].
OmiR-663 inhibits the proliferation, migration, and invasion of A172 cells by targeting TGF - β 1 [8].
OmiR-197 inhibits the proliferation of A172 cells by upregulating GAB2 expression [9].
7. Drug sensitivity and resistance
Chemotherapy drug sensitivity: A172 cells exhibit resistance to certain chemotherapy drugs (such as POH), which is related to their protein expression patterns and metabolic characteristics [5,14].
Radiosensitivity: A172 cells have a weaker response to gamma knife radiotherapy, but certain extracts (such as walnut shell extract) may enhance their radiosensitivity by inducing apoptosis or inhibiting invasion [3,13].
8. Research application
A172 cells are widely used for studying the molecular mechanisms, drug screening, and treatment strategies of glioblastoma.
Research has found that targeting the SKA1 gene can effectively inhibit the proliferation and invasion ability of A172 cells [10]; In addition, natural compounds such as Erianin have also shown inhibitory effects on A172 cells [12].
A172 cells were used to study the anti-tumor effect of Jinlong Capsule (JLC), and it was found that it significantly inhibited cell proliferation and migration by inhibiting the mTOR signaling pathway [2].
Aspirin induces apoptosis in A172 cells by blocking the IL-6-STAT3 signaling pathway.
Curcumin induces autophagy to mediate apoptosis in A172 cells [17].
9. Comparison with other cell lines
Compared with other glioblastoma cell lines such as U87MG and LN229, A172 cells exhibit different biological characteristics and drug responsiveness. For example, A172 cells have stronger resistance to POH and lower sensitivity to certain chemotherapy drugs such as Taxol [7,15].
A172 cells are an important tool for studying glioblastoma, and their unique biological characteristics and molecular mechanisms provide important clues for developing new therapeutic strategies.
References
1. TRPM7 channels regulate glioma stem cell through STAT3 and Notch signaling pathways. [PMID: 25192910]
2. Jinlong capsule inhibits migration and invasion in human glioblastoma cells via the modulation of mTOR/S6 signaling pathway. [PMID: 31114156]
3. The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth. [PMID: 33491752]
4. AT101-Loaded Cubosomes as an Alternative for Improved Glioblastoma Therapy. [PMID: 33116479]
5. A chemo-resistant protein expression pattern of glioblastoma cells (A172) to perillyl alcohol. [PMID: 20806975]
6. Aspirin induces apoptosis through the blockade of IL-6-STAT3 signaling pathway in human glioblastoma A172 cells. [PMID: 19595669]
7. Differential sensitivity of human glioblastoma LN18 (PTEN-positive) and A172 (PTEN-negative) cells to Taxol for apoptosis. [PMID: 18804099]
8. MicroRNA-663 inhibits the proliferation, migration and invasion of glioblastoma cells via targeting
TGF-β1. [PMID: 35506455]
9. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. LI QIANG TIAN et al. [PMID: 27035789]
10. Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells. [PMID: 25573192]
11. Notch-1 activation-dependent p53 restoration contributes to resveratrol-induced apoptosis in glioblastoma cells. [PMID: 21743969]
12. Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells. Archives of Biological Sciences 2022, 74(00):21-21
13. Radiosensitization Effects of a Zataria multiflora Extract on Human Glioblastoma Cells. [PMID: 26514525]
14. Dynamic proteomic overview of glioblastoma cells (A172) exposed to perillyl alcohol. [PMID: 20083244]
15. GBM Cells Exhibit Susceptibility to Metformin Treatment According to TLR4 Pathway Activation and Metabolic and Antioxidant Status. [PMID: 36765551]
16. Enhancement of SOX-2 expression and ROS accumulation by culture of A172 glioblastoma cells under
non-adherent culture conditions. [PMID: 26035068]
17.Curcumin-induced cell death depends on the level of autophagic flux in A172 and U87MG human glioblastoma cells [J]. Lee Jong-Eun et al.Chin J Nat Med, 2020, 18(2): 114-122.