Summary
| Production Name | GAPDH Monoclonal Antibody(2B8) |
| Application | WB,IF,IHC-p,Elisa |
| Reactivity | human;Rat;Mouse;Mk;Dg;Ch;Hamster;Rabbit;Pig;sheep;Insect;Yeast;Bovine |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Gene Name | GAPDH |
| Immunogen | Synthetic Peptide of GAPDH |
| Specificity | The antibody detects endogenous GAPDH protein. |
| Dilution Ratio | WB 1:5000-20000, IHC-P 1:200-300, IF-ICC 1:200 |
| Host | Monoclonal, Mouse |
| Isotype | |
| Buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Background | Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, etc. |
