Recombinant Rat TNF alpha

Recombinant Rat TNF alpha

Size1:10μg price1:$136
Size2:50μg price2:$378
Size3:500μg price3:$1890 SKU: PER1678 Category: Cytokines Tags: ,

Datasheet

Name

Recombinant Rat TNF alpha

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin level

<1 EU/µg as determined by LAL test.

Construction

Recombinant Rat Tumor Necrosis Factor is produced by our E.coli expression system and the target gene encoding Leu80-Leu235 is expressed.

Accession #

P16599

Host

E.coli

Species

Rat

Predicted Molecular Mass

17.4 KDa

Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Form

Lyophilized

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Stability&Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

 

 

Alternative Names

Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2

 

Background

Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

 

Note

For Research Use Only , Not for Diagnostic Use.

 

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