Recombinant Mouse SHH (C25II)-Cytokines-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

Name	Recombinant Mouse SHH (C25II)
Purity	Greater than 95% as determined by reducing SDS-PAGE
Endotoxin level	<0.01 EU/µg as determined by LAL test.
Construction	Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198(Cys25Ile-Ile) is expressed.
Accession #	Q62226
Host	E.coli
Species	Mouse
Predicted Molecular Mass	19.8 KDa
Buffer	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.
Form	Lyophilized
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Stability&Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Alternative Names

Sonic Hedgehog Protein; SHH; HHG-1; SHH

Background

Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N/xadterminal fragment (Shh/xadN), and a catalytic/xadprocessing,glycosylated, 34 kDa, 239 aa C/xadterminal fragment. The 20 kDa Shh/xadN fragment is the core of the active hedgehog molecule. Mouse Shh/xadN is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh/xadN, respectively.

Note

For Research Use Only , Not for Diagnostic Use.