Recombinant Human MIF(N-6His)

Recombinant Human MIF(N-6His)

Size1:10μg price1:$168
Size2:50μg price2:$465
Size3:500μg price3:$2325 SKU: PEH1164 Category: Cytokines Tags: ,

Datasheet

Name

Recombinant Human MIF(N-6His)

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin level

<1 EU/µg as determined by LAL test.

Construction

Recombinant Human Macrophage Migration Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Met1-Ala115 is expressed with a 6His tag at the N-terminus.

Accession #

P14174

Host

E.coli

Species

Human

Predicted Molecular Mass

14.6 KDa

Buffer

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 50% Glycerol, pH7.4.

Form

Liquid

Shipping

The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below.

Stability&Storage

Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

 

 

 

Alternative Names

Macrophage migration inhibitory factor; MIF; MMIF; Glycosylation-inhibiting factor; GLIF; L-dopachrome tautomerase; Phenylpyruvate tautomerase

 

Background

Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity.

 

Note

For Research Use Only , Not for Diagnostic Use.

 

Related products