CD33 (18N8) Rabbit Monoclonal Antibody

CD33 (18N8) Rabbit Monoclonal Antibody

Size1:50μL Price1:$128
Size2:100μL Price2:$230
Size3:200μL Price3:$380
Application:WB,IHC-P,ICC/IF,IP,IF-P

Reactivity:Human
Conjugate:Unconjugated
Optional conjugates: Biotin, FITC (free of charge).
See other 26 conjugates.

Gene Name:CD33
SKU: AMRe08370 Category: Rabbit Monoclonal Antibody Tags: , , , , , , ,

Datasheet

Summary

Production Name

CD33 (18N8) Rabbit Monoclonal Antibody

Description

Rabbit Monoclonal Antibody

Host

Rabbit

Application

WB,IHC-P,ICC/IF,IP,IF-P

Reactivity

Human

 

Performance

Conjugation

Unconjugated

Modification

Unmodified

Isotype

IgG

Clonality

Monoclonal

Form

Liquid

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Purification

Affinity purification

 

Immunogen

Gene Name

CD33

Alternative Names

CD33; gp67; My9; p67; SIGLEC3;

Gene ID

945

SwissProt ID

P20138

 

Application

Dilution Ratio

WB 1:1000, IHC-P/IF-P 1:100-1:200, ICC/IF 1:50, IP 1:20-1:50

Molecular Weight

40kDa

 

Background

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP- 2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).

 

Research Area