Cell Fluorescent Dye

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Cell Fluorescent Dyes

Cell Fluorescent Dyes are detection tools developed based on the principles of fluorescent chemistry, the characteristics of biomolecules, and the structural features of cells. They are specifically designed for labeling and observing various structures and molecules within cells. These dyes are widely used in cellular morphology, cell function analysis, cell signaling, live imaging, and multiplex labeling.

EnkiLife Cell Fluorescent Dyes, with their outstanding performance and diverse options, provide strong support for life science research.

Cytoskeleton Dyes

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Cytoskeleton fluorescent dyes are essential tools for labeling and visualizing the cytoskeleton structures within cells. They play a significant role in various fields such as cell morphology, cell function analysis, cell signaling, live imaging, and multiplex labeling. The cytoskeleton is composed of actin, tubulin, and intermediate filaments. These structures are crucial for maintaining cell shape, cell motility, cell division, and signal transduction.

Cat#Product NameExcitation WavelengthEmission Wavelength
RA20015Rhodamine-Phalloidin546nm575nm
RA20011Fluorescein 555-Phalloidin555nm565nm
RA20010Fluorescein 488-Phalloidin490nm515nm
RA20014Fluorescein 680-Phalloidin681nm698nm
RA20013Fluorescein 633-Phalloidin630nm650nm
RA20012Fluorescein 594-Phalloidin590nm617nm

Subcellular Structure Dyes

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Subcellular structure fluorescent dyes are fluorescent markers used to label and observe various organelles and structures within cells. They are of significant importance to cell biology research. These dyes provide powerful tools for cell biologists, enabling the visualization of different structures and functions within cells through appropriate selection and application.

Cat#Product NameExcitation WavelengthEmission Wavelength
RA20020Mitochondrial Tracker (Red)579nm599nm
RA20021Mitochondrial Tracker (Green)490nm523nm
RA20022JC-1 (Mitochondrial Membrane Potential Probe)550nm / 485nm600nm / 535nm
RA20024DASPEI (Mitochondrial Fluorescent Probe)461nm589nm
RA200254-Di-1-ASP (Mitochondrial Fluorescent Probe)474nm606nm
RA20026Lysosome Tracker (DeepRed)649nm665nm
RA20027Lysosome Tracker (Red)577nm590nm
RA20028Lysosome Tracker (Green)504nm511nm
RA20030Dihydroethidium (Hydroethidine;DHE)518nm610nm
RA20031Fluo-3 AM (2mM), Calcium Ion Fluorescent Probe506nm526nm
RA20032Fluo-4 AM (2mM), Calcium Ion Fluorescent Probe494nm516nm
RA20033Fluo-4 AM, Calcium Ion Fluorescent Probe494nm516nm
RA20034Fluo-3 AM, Calcium Ion Fluorescent Probe518nm610nm

Cell Membrane Dyes

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Cell membrane fluorescent dyes are fluorescent markers used to label cell membranes and other lipophilic biological structures. Common cell membrane fluorescent dyes include DiD, DiR, DiO, and DiI dyes, which are a group of lipophilic fluorescent dyes. Once these dyes enter the cell membrane, they laterally diffuse across the entire membrane, and at optimal concentrations, they can stain the entire cell membrane. These dyes come in different fluorescence colors, making them suitable for multicolor fluorescence imaging analysis and flow cytometry in live cells. They provide great convenience for the study of cell membrane morphology and structure.

Cat#Product NameExcitation WavelengthEmission Wavelength
RA20019Enhanced DiO Membrane Probe (Green)484nm501nm
RA20001DiA Membrane Probe (Green)456nm590nm
RA20002Dilinoleyl DiI Membrane Probe (Orange-Red)549nm565nm
RA20003DiD Membrane Probe (Red)644nm663nm
RA20004DiI Membrane Probe (Orange-Red)549nm565nm
RA20005DiO Membrane Probe (Green)484nm501nm
RA20006DiR Membrane Probe (Near-Infrared)748nm780nm
RA20007CM-DiI Membrane Probe (Orange-Red)549nm565nm
RA20008CM-DiD Membrane Probe (Red)644nm663nm
RA20009CM-DiD Membrane Probe (Orange-Red)549nm565nm

Nuclear Dyes

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Nuclear fluorescent dyes are a class of dyes that can bind to DNA in the cell nucleus and emit fluorescence. They play an important role in cell biology research. Commonly used nuclear fluorescent dyes include DAPI, Hoechst 33258, Hoechst 33342, PI (Propidium Iodide), and others. These nuclear fluorescent dyes provide a variety of options for observing and studying the cell nucleus due to their distinct fluorescence and staining properties.

Cat#Product NameExcitation WavelengthEmission Wavelength
RA20035DAPI Nuclear Staining Solution (Ready-to-use)360nm460nm
RA20036DAPI Nuclear Dye360nm460nm
RA20037Hoechst 33342 Nuclear Staining Solution (Ready-to-use)346nm / 350nm460nm / 461nm
RA20038Hoechst 33258 Nuclear Staining Solution (Ready-to-use)346nm / 352nm460nm / 461nm
RA20039Hoechst 33342 Live Cell DNA Dye346nm / 350nm460nm / 461nm
RA20040Hoechst 33258 Live Cell DNA Dye346nm / 352nm460nm / 461nm
RA20041Propidium Iodide (PI)493nm / 535nm636nm / 617nm
RA20042dsDNA Quantification Reagent (Green)480nm520nm
RA20067DRAQ5 Live Cell DNA Staining Solution647nm681nm
RA20068High Sensitivity dsDNA Quantification Kit485nm530nm
RA200165(6)-CFDA SE Live Cell Fluorescent Probe490-500nm517-520nm
RA200176-CDCFDA SE Live Cell Fluorescent Probe504nm529nm

Product Advantages

1. High Specificity and Sensitivity
  • Specific Binding: Cell fluorescent dyes can specifically bind to target cell structures or molecules, such as cell membranes, nuclei, mitochondria, lysosomes, etc. This ensures that the signal originates only from the target area, with minimal background fluorescence.
  • High Sensitivity: The intensity of the fluorescent signal is proportional to the concentration or quantity of the target molecules. Even at low concentrations, the signal can be detected by high-sensitivity microscopes or imaging devices.
2. Low Toxicity and High Stability
  • Low Cytotoxicity: Optimized chemical design ensures minimal impact on cellular physiological functions, making the dyes suitable for long-term live-cell imaging.
  • High Stability: The fluorescent signal remains stable under various experimental conditions, such as different pH levels, temperatures, and light exposure, with minimal quenching.
3. Diverse Fluorescence Wavelengths
  • Wide Wavelength Range: Cell fluorescent dyes cover a broad range of wavelengths from visible to near-infrared light (400-750 nm), meeting the needs of both single-color and multiplex labeling experiments.
  • Low Background Fluorescence: Near-infrared fluorescent dyes (e.g., 690-750 nm) exhibit lower background fluorescence and higher tissue penetration capabilities, making them ideal for live imaging.
4. Easy Operation and Strong Compatibility
  • Easy to Use: Most cell fluorescent dyes have simple application methods with minimal staining steps and no complex pre-treatment required.
  • Strong Compatibility: These dyes are compatible with common cell fixatives, permeabilization agents, and other fluorescent markers, allowing seamless integration into various experimental workflows.