Ham's F-10 Nutrient Mixture is a culture medium designed by Ham in 1963 for serum-free culture of CHO cells. Ham's F-10 contains rich nutrients and is suitable for the culture of human diploid cells, cell cloning culture under low serum conditions, normal culture of mammalian cells, chromosome analysis of leukocytes, and primary cell culture of rats, rabbits, chickens and other animals.Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. This product is only used for research or further research, not for diagnosis and treatment.
