Ham's F-10 Nutrient Mixture is a culture medium designed by Ham in 1963 for serum-free culture of CHO cells. Ham's F-10 contains rich nutrients and is suitable for the culture of human diploid cells, cell cloning culture under low serum conditions, normal culture of mammalian cells, chromosome analysis of leukocytes, and primary cell culture of rats, rabbits, chickens and other animals.HEPES is an excellent biological buffer with no toxic effect on cells. The culture medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. This product is only used for research or further research, not for diagnosis and treatment.