Ham's F-10 (With HEPES)

Ham's F-10 (With HEPES)

Size1:500ml Price1:$60 SKU: CMB0087 Category: Cell Medium Tags:

Datasheet

Ham's F-10 Nutrient Mixture is a culture medium designed by Ham in 1963 for serum-free culture of CHO cells. Ham's F-10 contains rich nutrients and is suitable for the culture of human diploid cells, cell cloning culture under low serum conditions, normal culture of mammalian cells, chromosome analysis of leukocytes, and primary cell culture of rats, rabbits, chickens and other animals.HEPES is an excellent biological buffer with no toxic effect on cells. The culture medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.

 

Form

Liquid

Size

500mL

Concentration

pH

7.2~7.4

L-Glutamine

1mM

NaHCO3

1200mg/L

D-Glucose

1100mg/L

HEPES

25mM

Phenol Red

1.2mg/L

Transportation

Room temperature

Storage

Store at 2-8°C away from light

Shelf Life

12 months

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Precautions

1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;

2. This product is only used for research or further research, not for diagnosis and treatment.