McCoy's 5A medium was designed by Thomas McCoy and others in 1959. It was originally used specifically for the culture of Novikoff Hepatoma cells. McCoy's 5A medium is a modified Basal Medium 5A. Unlike other culture media, McCoy's 5A contains reduced glutathione, bacterial peptone, and a high concentration of glucose. McCoy's 5A is widely used for the culture of various types of primary cells, such as bone marrow, skin, gums, kidneys, spleen, lungs, rat embryos, omentum, etc. In addition, McCoy's 5A medium is also used for tissue biopsy culture, cell line establishment, and the culture of some lymphocytes and cells that are difficult to culture.HEPES is an excellent biological buffer with no toxic effect on cells. The culture medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. This product is only used for research or further research, not for diagnosis and treatment.
