DMEM/F12 (Without Phenol Red)

DMEM/F12 (Without Phenol Red)

Size1:500ml Price1:$35 SKU: CMB0060 Category: Cell Medium Tags:

Datasheet

DMEM/F12 medium is a mixture of DMEM and Ham's F12 medium in a 1:1 ratio. It contains a variety of trace elements and is widely used in the culture of mammalian cells such as MDCK, glial cells, fibroblasts, human endothelial cells and rat fibroblasts.Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.

 

Form

Liquid

Size

500mL

Concentration

pH

7.2~7.4

L-Glutamine

2.5mM

NaHCO3

2438mg/L

D-Glucose

3151mg/L

Sodium Pyruvate

0.5mM

HEPES

Without

Phenol Red

Without

Transportation

Room temperature

Storage

Store at 2-8°C away from light

Shelf Life

12 months

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Precautions

1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;

2. This product is only used for research or further research, not for diagnosis and treatment.