DMEM/F12 medium is a mixture of DMEM and Ham's F12 medium in a 1:1 ratio. It contains a variety of trace elements and is widely used in the culture of mammalian cells such as MDCK, glial cells, fibroblasts, human endothelial cells and rat fibroblasts.HEPES is an excellent biological buffer with no toxic effect on cells. The culture medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. This product is only used for research or further research, not for diagnosis and treatment.
