RPMI 1640 medium is named after the Roswell Park Memorial Institute (RPMI), where it was developed, and 1640 is the code name of the medium. RPMI-1640 medium was originally designed for lymphocyte culture, but is now widely used in the culture of various normal cells and cancer cells, especially suspension cells, and is one of the most widely used culture media. This product contains a variety of ingredients such as amino acids, vitamins, and inorganic salts required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product must be used with serum or serum-free supplements.Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. This product is only used for research or further research, not for diagnosis and treatment.
