Urine Protein Assay Kit (Coomassie Brilliant Blue Method)

Urine Protein Assay Kit (Coomassie Brilliant Blue Method)

Size1:100T  Price1:$240

SKU: BC00052 Category: Metabolism Kits Tags: ,

Datasheet

Product Introduction

Normally, the amount of protein in urine is very low and usually cannot be detected by routine urine testing methods. However, certain diseases or abnormal physical conditions may cause increased protein in the urine, which is called proteinuria.

 

Principle

Coomassie brilliant blue G-250 is red in the free state, with the maximum light absorption at 465nm; when it binds to protein, it turns cyan, and the protein-pigment conjugate has the maximum light absorption at a wavelength of 595 nm. The depth of its color is proportional to the protein content. The figure below shows the standard curve of this kit for detecting urine protein.

Components

No.

Components

Size (100T)

Storage

Reagent 1

Chromogen Stock Solution

15 mL

 -20, protect from light.

Reagent 2

1 mg Standard

1 mg × 2 vials

-20, protect from light.

Consumable 1

Microplate

1 plate

RT

Consumable 2

Plate Sealer

2 pieces

RT

 

Storage

The unopened kit can be stored at -20°C for 6 months.


Notes

This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.