GMP Recombinant Human FGF-4-GMP Cytokines-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

Product Name	GMP Recombinant Human FGF-4
Full Name	FGF-4
Purity	Greater than 95% as determined by reducing SDS-PAGE
Endotoxin level	≤10 EU/mg
Construction	Recombinant Human FGF-4 is produced by our Mammalian cell expression system and the target gene encoding Ala31-Leu206 is expressed.
Accession #	P08620
tag	Tag free
Host	Mammalian cell
Species	Human
PredictedMolecularMass	19.3 kDa
Form	Lyophilized
Buffer	PBS,5% mannitol and 0.01% Tween 80, pH7.4
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Stability&Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Alternative Names

Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3

Bioactivity

The ED₅₀ for this effect is 0.25-1 ng/mL

Background

Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.

Note

For Research Use Only , Not for Diagnostic Use.