Summary
| Production Name | PRAS40 Rabbit Monoclonal Antibody |
| Description | Recombinant Rabbit Monoclonal antibody |
| Host | Rabbit |
| Application | WB |
| Reactivity | Human,Rat |
Performance
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | IgG |
| Clonality | Monoclonal Antibody |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification | Affinity Purified |
Immunogen
| Gene Name | AKT1S1 |
| Alternative Names | AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate |
| Gene ID | 84335 |
| SwissProt ID | Q96B36 |
Application
| Dilution Ratio | WB: 1/500-1/1000 |
| Molecular Weight | Calculated MW: 27 kDa; Observed MW: 40 kDa |
Background
PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
Research Area
Cell Biology
