Crotonyl-Histone H2B (Lys12) Rabbit Monoclonal Antibody

Crotonyl-Histone H2B (Lys12) Rabbit Monoclonal Antibody

Size1:50μl Price1:$188
Size2:100μl Price2:$338
Size3:500μl Price3:$1200
SKU: AMRe03903 Category: Rabbit Monoclonal Antibody Tags: , , ,

Datasheet

Summary

Production Name

Crotonyl-Histone H2B (Lys12) Rabbit Monoclonal Antibody

Description

Recombinant Rabbit Monoclonal antibody

Host

Rabbit

Application

WB

Reactivity

Human, Mouse, Rat

 

Performance

Conjugation

Unconjugated

Modification

Crotonylated

Isotype

IgG

Clonality

Monoclonal Antibody

Form

Liquid

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Purification

Affinity Purified

 

Immunogen

Gene Name

H2BC4

Alternative Names

H2BK11cr, Histone H2B.1 A;Histone H2B.a (H2B/a);Histone H2B.g (H2B/g);Histone H2B.h (H2B/h);Histone H2B.k (H2B/k);Histone H2B.l (H2B/l)

Gene ID

3018

SwissProt ID

P33778

 

Application

Dilution Ratio

WB: 1/500-1/1000

Molecular Weight

Calculated MW:14 kDa;Observed MW: 14 kDa

 

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys11 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

 

Research Area

Epigenetics and Nuclear Signaling