MonoMethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody

MonoMethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody

Size1:50μl Price1:$188
Size2:100μl Price2:$338
Size3:500μl Price3:$1200
SKU: AMRe03910 Category: Rabbit Monoclonal Antibody Tags: , , ,

Datasheet

Summary

Production Name

MonoMethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody

Description

Recombinant Rabbit Monoclonal antibody

Host

Rabbit

Application

WB

Reactivity

Human, Mouse, Rat

 

Performance

Conjugation

Unconjugated

Modification

Monomethylated

Isotype

IgG

Clonality

Monoclonal Antibody

Form

Liquid

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Purification

Affinity Purified

 

Immunogen

Gene Name

H3C1

Alternative Names

H3K9me; Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f

Gene ID

8350

SwissProt ID

P68431

 

Application

Dilution Ratio

WB: 1/500-1/1000

Molecular Weight

Calculated MW:15 kDa;Observed MW: 17 kDa

 

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors.

 

Research Area

Epigenetics and Nuclear Signaling