PMS2 Rabbit Polyclonal Antibody

PMS2 Rabbit Polyclonal Antibody

Size1:50μl Price1:$168
Size2:100μl Price2:$300
Size3:500μl Price3:$1200
SKU: APRab00008 Category: Polyclonal Antibody Tags: , , , ,

Datasheet

Summary

Production Name

PMS2 Rabbit Polyclonal Antibody

Description

Primary antibody

Host

Rabbit

Application

WB,IHC

Reactivity

Human

 

Performance

Conjugation

Unconjugated

Modification

Unmodified

Isotype

IgG

Clonality

Polyclonal Antibody

Form

Liquid

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purification

Affinity Chromatography

 

Immunogen

Gene Name

PMS2

Alternative Names

DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2

Gene ID

5395

SwissProt ID

P54278

 

Application

Dilution Ratio

WB: 1/500-1/1000 IHC: 1/50-1/100

Molecular Weight

Calculated MW: 96 kDa; Observed MW: 110 kDa

 

Background

Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.

 

Research Area

Epigenetics and Nuclear Signaling