Summary
| Production Name | Topoisomerase I Rabbit Polyclonal Antibody |
| Description | Primary antibody |
| Host | Rabbit |
| Application | WB,IHC |
| Reactivity | Human,Mouse |
Performance
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | IgG |
| Clonality | Polyclonal Antibody |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification | Affinity Chromatography |
Immunogen
| Gene Name | TOP1 |
| Alternative Names | TOP1; DNA topoisomerase 1; DNA topoisomerase I |
| Gene ID | 7150 |
| SwissProt ID | P11387 |
Application
| Dilution Ratio | WB: 1/500-1/1000 IHC: 1/50-1/100 |
| Molecular Weight | Calculated MW: 91 kDa; Observed MW: 91 kDa |
Background
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
Research Area
Epigenetics and Nuclear Signaling
