| ◤Test Principle：
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT4. During the reaction, fT4 in the sample or standard competes with a fixed amount of fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to fT4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of fT4 in tested samples can be calculated by comparing the OD of the samples to the standard curve. ◤Storage：Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |
◤Key Features:
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| ◤Assay Procedures: |
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| ◤Typical Data: | |
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fT4 ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
| ◤Precision: | ||||||||||||||||||||||||||||||||||||||||||
| Intra-assay precision (intra-assay precision): Low, medium, and high levels of fT4 were measured on a single plate 20 times at each level. Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of fT4 were assayed 20 times per plate on 3 different plates. | ||||||||||||||||||||||||||||||||||||||||||
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| ◤Recovery: | ||||||||||||
| Three different levels of fT4 spiked in samples were evaluated for recovery in different matrices. | ||||||||||||
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| ◤Linearity: |
| A high concentration of fT4 is added to the sample, which is then diluted with a standard and a sample diluent to bring the value of the sample within the detection range. |
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| ◤Component List: | ||||||||||||||||||||||||||
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