| ◤Test Principle：
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 25-HVD3. During the reaction, 25-HVD3 in the sample or standard competes with a fixed amount of 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve. ◤Storage：Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |
◤Key Features:
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| ◤Assay Procedures: |
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| ◤Typical Data: | |
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25-HVD3 ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
| ◤Precision: | ||||||||||||||||||||||||||||||||||||||||||
| Intra-assay precision (intra-assay precision): Low, medium, and high levels of 25-HVD3 were measured on a single plate 20 times at each level. Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of 25-HVD3 were assayed 20 times per plate on 3 different plates. | ||||||||||||||||||||||||||||||||||||||||||
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| ◤Recovery: | ||||||||||||
| Three different levels of 25-HVD3 spiked in samples were evaluated for recovery in different matrices. | ||||||||||||
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| ◤Linearity: |
| A high concentration of 25-HVD3 is added to the sample, which is then diluted with a standard and a sample diluent to bring the value of the sample within the detection range. |
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| ◤Component List: | ||||||||||||||||||||||||||
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