Alpha-MEM, Nucleoside-Free (with Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Earle's Salts)

Alpha-MEM, Nucleoside-Free (with Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Earle's Salts)

Size1:2L Price1:$20 SKU: CMB0038 Category: Cell Medium Tags:

Datasheet

MEM medium (Minimum Essential Medium) is also called the minimum essential medium, minimum basic medium or low-limit Eagle medium. It was developed by Harry Eagle based on Eagle Basic Medium (BEM). It is the most basic and widely applicable medium and one of the most commonly used mediums in animal cell culture. MEM medium contains only 12 essential amino acids, glutamine and 8 vitamins. It has simple ingredients and is mainly used for the culture of adherent cells. It can also be used for other types of cell culture after the formula is modified. MEM medium containing NEAA (non-essential amino acids) is based on MEM medium and adds 7 NEAAs: L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine. It can reduce the side effects of the production of non-essential amino acids by cells themselves during cell culture and effectively promote cell proliferation and metabolism.

 

Earle's Balanced Salt Solution (EBSS) is one of the most commonly used phosphate buffers. Its main components include NaCl, KCl, Na2HPO4, NaHCO3, etc. It has the functions of maintaining osmotic pressure, keeping pH stable, and providing simple nutrition.

 

 

 

Details

Form

Powder

Size

2L

L-Glutamine

With

NaHCO3

Without

D-Glucose

1g/L

Sodium Pyruvate

With

HEPES

Without

Phenol Red

With

Balanced Salt Solution

Earle's

Nucleoside

Without

NEAA

With

Transportation

Room temperature

Storage

Store at 2-8°C away from light

Shelf Life

24 months

 

 

Precautions

1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;

2. This product is only used for research or further research, not for diagnosis and treatment.

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