eIF4E (8C8) Mouse Monoclonal Antibody-Mouse Monoclonal Antibody-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

eIF4E (8C8) Mouse Monoclonal Antibody

Summary

Production Name	eIF4E (8C8) Mouse Monoclonal Antibody
Description	Primary antibody
Host	Mouse
Application	WB
Reactivity	Human,Mouse,Rat

Performance

Conjugation	Unconjugated
Modification	Unmodified
Isotype	IgG1
Clonality	Monoclonal Antibody
Form	Liquid
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Buffer	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Purification	Affinity Purified

Immunogen

Gene Name	EIF4E
Alternative Names	EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Gene ID	1977
SwissProt ID	P06730

Application

Dilution Ratio	WB: 1/500-1/1000
Molecular Weight	Calculated MW: 25 kDa; Observed MW: 25 kDa

Background

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Research Area

Epigenetics and Nuclear Signaling