| ◤Test Principle：
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve. ◤Storage：Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |
◤Key Features:
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| ◤Assay Procedures: |
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| ◤Typical Data: | |
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Human LEP ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
| ◤Precision: | ||||||||||||||||||||||||||||||||||||||||||
| Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human LEP were measured on a single plate 20 times at each level. Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human LEP were assayed 20 times per plate on 3 different plates. | ||||||||||||||||||||||||||||||||||||||||||
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| ◤Recovery: | ||||||||||||
| Three different levels of Human LEP spiked in samples were evaluated for recovery in different matrices. | ||||||||||||
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| ◤Linearity: |
| A high concentration of Human LEP is added to the sample, which is then diluted with a standard and a sample diluent to bring the value of the sample within the detection range. |
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| ◤Component List: | ||||||||||||||||||||||||||
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