Full Name

Meprin A Alpha

Synonym

PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

Assay Type

Competitive

Reactivity

Mouse

Range

49.4-4000pg/mL

Sensitivity

19.1pg/mL

Sample Type

Serum,plasma and other biological fluids

UNIProt ID

Microplate

96-wells plate breakable into 12 x 8 wells strip  

◤Test Principle：

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse MEP1a. During the reaction, Mouse MEP1a in the sample or standard competes with a fixed amount of Mouse MEP1a on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MEP1a. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse MEP1a in tested samples can be calculated by comparing the OD of the samples to the standard curve.

◤Storage：

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.