◤Key Features: |
|
Full Name |
Testosterone |
Synonym |
T |
Assay Type |
Competitive |
Reactivity |
Universal |
Range |
0.37-30ng/mL |
Sensitivity |
152.5ng/mL |
Sample Type |
Serum,Plasma,Urine,Saliva |
UNIProt ID |
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Microplate |
96-wells plate breakable into 12 x 8 wells strip |
◤Test Principle: |
|
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T. During the reaction, T in the sample or standard competes with a fixed amount of T on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
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◤Storage: |
|
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |