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◤Key Features: |
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Full Name |
4-Hydroxynonenal |
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Synonym |
4-HNE |
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Assay Type |
Competitive |
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Reactivity |
Universal |
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Range |
0.63-40ng/mL |
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Sensitivity |
0.38ng/mL |
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Sample Type |
Serum,plasma and other biological fluids |
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UNIProt ID |
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Microplate |
96-wells plate breakable into 12 x 8 wells strip |
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◤Test Principle： |
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
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◤Storage： |
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Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |
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