Product Introduction

G6PDH catalyzes the conversion of glucose-6-phosphate (G6P) into 6-phosphogluconolactone. ate, 6-PG), which is the first step of the pentose phosphate pathway (PPP) and the rate-limiting step of the pathway. The pentose phosphate pathway is essential for the production of NADPH (reduced nicotinamide adenine dinucleotide phosphate, also known as reduced coenzyme II) and pentoses. NADPH is essential for regulating redox balance and fatty acid biosynthesis through the regeneration of GSH. Therefore, the deficiency of G6PDH can lead to some diseases caused by the inability to generate NADPH, such as neonatal jaundice, non-immune hemolytic anemia, etc.

Product Features

1.WST-8 is an upgraded substitute for MTT, and has obvious advantages over MTT or other MTT-like products such as XTT, MTS, etc. First, the formazan generated by the reduction of MTT by some dehydrogenases is not water-soluble and requires a specific dissolving solution to dissolve; while the formazan generated by WST-8, XTT, and MTS are all water-soluble, which can save the subsequent dissolution step. Secondly, the formazan generated by WST-8 is more soluble than the formazan generated by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, making the experimental results more stable.

2.Compared with MTT, XTT, etc., WST-8 has a wider linear range and higher sensitivity.

3.Compared with WST-1, WST-8 has higher detection sensitivity, is more soluble, and is more stable.

4.This kit is easy to use, highly sensitive, and has a wide linear range. It can detect G6PDH with a content as low as 0.05mU per well, and a good linear relationship between 1mU/ml (0.05mU/well) and 500mU/ml (25mU/well). The detection can be performed using lysate of cells, tissues, etc., without the need to separate and purify G6PDH in cells, tissues, or other samples.

 Principle

G6P is oxidized to generate 6-PG under the action of G6PDH. In this reaction process, NADP+ is reduced to NADPH. The generated NADPH reduces WST-8 to generate orange-yellow formazan under the action of the electron coupling reagent 1-mPMS (1-Methoxy-5-methylphenaziniumMethylSulfate), with a maximum absorption peak at around 450nm. The formazan generated in the reaction system is directly proportional to the activity of G6PDH in the sample. The principle of WST-8 method for detecting G6PDH is shown in the figure below:

Components

Storage

The unopened kit can be stored at -20°C for 12 months.

Notes

This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.