Product Introduction
Glutamate is present in large quantities in cereal proteins and in high concentrations in animal brains. Glutamate plays an important role in protein metabolism in living organisms and is involved in many important chemical reactions in animals, plants and microorganisms. It is also the most abundant excitatory neurotransmitter in the nervous system of vertebrates. Glutamate is an important non-essential amino acid, which means that the human body can synthesize enough nutrients for its use.
Features
1.WST-8 is an upgraded substitute for MTT, and has obvious advantages over MTT or other MTT-like products such as XTT, MTS, etc. First, the formazan generated by the reduction of MTT by some dehydrogenases is not water-soluble and requires a specific dissolving solution to dissolve; while the formazan generated by WST-8, XTT, and MTS are all water-soluble, which can save the subsequent dissolution step. Secondly, the formazan generated by WST-8 is more soluble than the formazan generated by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, making the experimental results more stable.
2.Compared with MTT, XTT, etc., WST-8 has a wider linear range and higher sensitivity.
3.Compared with WST-1, WST-8 has higher detection sensitivity, is more soluble, and is more stable.
Detection Principle
Under the catalytic action of enzymes, glutamic acid (Glu) can reduce NAD+ to NADH. NADH, under the action of hydrogen-donating substances, reduces WST-8 to form an orange-yellow substance, which has the maximum absorption peak at 470 nm. Within a certain range, the absorbance value is directly proportional to the concentration. By measuring the absorbance at 470 nm or nearby wavelengths, the content of Glu in the sample can be calculated.
Product Composition
Serial Number | Product Name | Packing specification ( 50T ) | Storage |
Reagent 1 | Buffer | 8 mL | -20℃, keep away from light |
Reagent 2 | Enzyme reagents | powder | -20℃, keep away from light |
Reagent 3 | Substrate | powder | -20℃, keep away from light |
Reagent 4 | Color developer | 1.2 mL | -20℃, keep away from light |
Reagent 5 | 50 mmol/L standard | 1 mL | -20℃, keep away from light |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage conditions
The unopened kit can be stored at -20°C for 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.
