Product Introduction
Glutathione is a small peptide composed of three amino acid residues. Its full name is glutamyl-cysteinyl-glycine, Since the thiol (SH) on cysteine is the active group of glutathione, it is often abbreviated as G-SH or GSH. Glutathione includes two forms: reduced glutathione (commonly known as GSH) and oxidized glutathione (oxidized glutathione disulfide). Since oxidized glutathione is formed by the dehydrogenation of two GSH groups, it is often abbreviated as GSSG or GSSG. Reduced glutathione is the main source of thiol groups in most living cells. It plays an important role in maintaining the proper redox state of thiol groups in proteins and is a key antioxidant in animal cells. Usually 90-95% of total glutathione is reduced glutathione.
Product Features
This kit provides protein removal reagent S, which can more accurately determine the amount of total glutathione in samples containing protein. The detection limit of this kit is 1μM. The actual measurement effect of the standard product is shown in the figure below:
Principle
Glutathione reductase can reduce oxidized glutathione (GSSG) to generate reduced glutathione (GSH), and GSH can react with the chromogenic substrate DTNB to produce yellow TNB and GSSG, and the amount of TNB generated can be detected by measuring A412. When the reaction system is properly set up and the two reactions are combined, the total glutathione (GSSG+GSH) is equivalent to a rate-limiting factor for color production, and the amount of total glutathione determines the amount of yellow TNB formed. Therefore, the amount of total glutathione can be calculated by measuring A412. The specific reaction principle of this kit is as follows:
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | Total Glutathione Assay Buffer | 60ml | -20℃ |
Reagent 2 | Glutathione reductase | 150μl | -20℃ |
Reagent 3 | Reduced Glutathione (GSH ) | 4.5mg | -20℃, prepare into solution,store at -20ºC after aliquoting. |
Reagent 4 | DTNB | 4.5mg | -20℃, prepare into solution, store at -20ºC after aliquoting. |
Reagent 5 | Protein Removal Reagent S | 0.4g | -20℃, prepare solution and store at 2-8 ºC. |
Reagent 6 | NADPH | 4mg | At -20℃, NADPH is dissolved and packaged,stored at -70ºC. It can be stored at 2-8 ºC for one day. After being stored at -20℃ for one week, NADPH will degrade by more than 10%. |
Reagent 7 | DMSO | 1.5ml | -20℃ |
Consumable 1 | Microplate(96 wells) | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
