Product Introduction
Glutathione is a small peptide composed of three amino acid residues. Its full name is glutamyl-cysteinyl-glycine, and its English name is glutamyl-cysteinylglycine, or glutathione for short. Since the thiol (SH) on cysteine is the active group of glutathione, it is often abbreviated as G-SH or GSH. Glutathione includes two forms: reduced glutathione (commonly known as GSH) and oxidized glutathione (oxidized glutathione disulfide). Since oxidized glutathione is formed by the dehydrogenation of two GSH groups through thiol groups, it is often abbreviated as G-S-S-G or GSSG. Reduced glutathione is the main source of thiol groups in most living cells. It plays an important role in maintaining the proper redox state of thiol groups in proteins and is a key antioxidant in animal cells. Usually 90-95% of total glutathione is reduced glutathione.
Product Features
This kit provides protein removal reagent S, which can more accurately determine the amount of reduced glutathione in samples containing protein. The actual measurement effect of the standard product is shown in the figure below :
Principle
GSH can react with the chromogenic substrate DTNB to produce yellow TNB and GSSG, and the amount of TNB generated can be detected by measuring A412. Therefore, the amount of reduced glutathione can be calculated by measuring A412. The specific reaction principle of this kit is as follows:
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | Reduced Glutathione Assay Buffer | 60ml | -20℃ |
Reagent 2 | Reduced Glutathione (GSH ) | 4.5mg | -20℃, prepare into solution, store at -20ºC after aliquoting. |
Reagent 3 | DTNB | 4.5mg | -20℃, prepare into solution, store at -20ºC after aliquoting. |
Reagent 4 | Protein Removal Reagent S | 0.4g | -20℃, prepare solution and store at 2-8 ºC. |
Reagent 5 | DMSO | 1.5ml | -20℃ |
Consumable 1 | Microplate(96 wells) | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
