Product Introduction
Glutathione reductase is distributed in many tissues and can maintain sufficient reduced glutathione (GSH) levels in cells.
Principle
Glutathione reductase can reduce oxidized glutathione (GSSG) to generate reduced glutathione (GSH), and GSH can react with the chromogenic substrate DTNB to produce yellow TNB and GSSG, and the amount of TNB generated can be detected by measuring A412. When the reaction system is properly set up and the two reactions are combined, when GSSG/GSH is excessive and GR is relatively insufficient, the glutathione reductase in the reaction system becomes the rate-limiting factor of the entire reaction system. At this time, the amount of yellow TNB generated is linearly positively correlated with the activity of glutathione reductase. Therefore, the activity level of glutathione reductase can be calculated by measuring A412. The specific reaction principle of this kit is as follows:
The detection effect of this kit on 37 μg liver sample is shown in the figure below:
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | Glutathione reductase assay buffer | 50ml | -20℃ |
Reagent 2 | Sample diluent | 50ml | -20℃ |
Reagent 3 | NADPH | 5mg | -20℃, after NADPH is dissolved, it should be appropriately divided and stored at -70ºC. It can be stored at 4ºC for one day. After being stored at -20ºC for one week, NADPH will degrade by more than 10%. |
Reagent 4 | Oxidized glutathione (GSSG) | 14.2mg | -20℃, GSSG is prepared into solution and stored at -20ºC after packaging. |
Reagent 5 | DTNB | 4.5mg | -20℃, DTNB is prepared into solution and stored at -20ºC after aliquoting. |
Reagent 6 | DMSO | 1.5ml | -20℃ |
Consumable 1 | Microplate(96 wells) | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
Unopened kits can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
