Product Introduction
Hydroxyl free radical is a highly active oxygen free radical with a very high redox potential. It can react with a variety of molecules in the body, causing cell damage and dysfunction. Therefore, the generation and removal of hydroxyl free radicals play a vital role in maintaining the health of organisms and preventing and treating related diseases. Hydroxyl free radical scavenging ability detection has a wide range of application value as an important means to evaluate the effects of antioxidants, study antioxidant mechanisms, and develop new drugs.
Detection principle
H 2 O 2 /Fe 2+ generates hydroxyl free radicals through Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radicals and react with them to generate the colored substance 2,3-dihydroxybenzoic acid, which has a characteristic absorption peak at 510 nm. After adding substances with scavenging ability, the colored substance decreases, so the ability of the sample to scavenge hydroxyl free radicals can be judged based on the absorbance value.
The standard was diluted with distilled water at different times, and then its hydroxyl free radical elimination ability was measured. The curve was drawn as shown in the left figure below, and the linear comparison range was obtained: 10-50% (data is for reference only). The reciprocal of the standard concentration and the hydroxyl free radical elimination rate was obtained to obtain a linear relationship as shown in the right figure below.
Product Composition
Serial Number | Product Name | Packing Specifications (100T) | Storage |
Reagent 1 | Substrate A | 30 mL | -20℃ |
Reagent 2 | Substrate B | 20 mL | -20℃, avoid light , store at 2-8℃ after opening , valid for three months. |
Reagent 3 | Substrate C | 60 mL | -20℃ |
Reagent 4 | Ascorbic acid standards | 28 mg | -20℃ |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 6 months. After opening, it can be stored at 2-8℃ for 3 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
