Product Introduction
Lipid peroxides are peroxides produced by unsaturated fatty acid chains after being acted upon by free radicals or reactive oxygen species. Under pathological conditions, the enhanced lipid peroxidation reaction can lead to an increase in the originally low LPO content. An increase in LPO content can damage the structure and function of cells, and LPO content is closely related to the body's immune system and aging.
Features
This kit can measure LPO in the range of 1-200 μM. The figure below shows the A532 readings of different concentrations of the standard.
Detection principle
LPO is heated under acidic conditions to produce malondialdehyde, which condenses with thiobarbituric acid (TBA) to produce a reddish-brown substance, trimethoate (3,5,5-trimethyloxazole-2,4-dione), with a maximum absorption wavelength of 532 nm. The LPO content in the sample can be estimated by colorimetry.
Product composition
Serial Number | Product Name | Packing Specifications ( 100T ) | Storage |
Reagent 1 | TBA | 25 mg | -20℃, keep away from light, after opening the bottle can be stored at room temperature or 2-8 degrees for three months. |
Reagent 2 | TBA preparation solution | 6.76 ml | -20℃, after opening the bottle can be stored at room temperature or 2-8 degrees for three months. |
Reagent 3 | TBA diluent | 15 ml | -20℃, after opening the bottle can be stored at room temperature or 2-8 degrees for three months. |
Reagent 4 | Antioxidants | 0.3 ml | -20℃, keep away from light. |
Reagent 5 | Standard (1 mM ) | 0.2 ml | -20℃ |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
