Product Introduction
This kit detects the activity of peroxidase (POD) in cells, tissues or other samples through colorimetric reaction. Peroxidase is a common enzyme that plays an important role in redox in organisms and is widely distributed in the liver, kidneys and red blood cells.
Principle
Under conditions where hydrogen peroxide is relatively abundant, peroxidase can catalyze the decomposition of hydrogen peroxide into water and oxygen. Hydrogen peroxide can oxidize a chromogenic substrate under the catalysis of peroxidase, resulting in a red product with a maximum absorption wavelength of 532nm. By measuring the absorbance at A532 or a similar wavelength and comparing it with a standard curve made from peroxidase standards, the peroxidase activity in the sample can be calculated.
The figure below shows the A532 readings for peroxidase standards detected by this kit.
Components
Components | Size (100T) | Storage |
Peroxidase Assay Buffer | 60ml | -20℃, can also be stored at 4℃ after opening. |
Hydrogen Peroxide (approximately 1M) | 5ml | -20℃ |
Chromogenic Substrate | 20ml | -20℃ |
Peroxidase (50U/ml) | 200μl | -20℃ |
Microplate | 1 plate | RT |
Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20°C for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
