Product Introduction
GPx is an important antioxidant enzyme that can eliminate harmful substances such as hydrogen peroxide in the human body, protect cells from free radical damage, and maintain the stability of the intracellular environment. Lipids in cells are prone to react with free radicals to produce lipid peroxides. Glutathione Peroxidase can use reduced glutathione (GSH) to reduce lipid peroxides, thereby eliminating the toxic effects of free radicals. Glutathione Peroxidase is distributed in almost all tissues. In some pathological conditions,the activity of Glutathione Peroxidase will be significantly up-or down-regulated.
Most GPx in cells contain selenium, and selenium is a component of the active center of the enzyme. There is also a small amount of GPx that does not contain selenium in cells. This kit detects the content of the most common selenium-containing GPx.
Product Features
The organic peroxide reagent (t-Bu-OOH) provided in this kit does not react with GS in the absence of Glutathione Peroxidase, nor is it catalyzed and decomposed by cellular catalase. Therefore, it can relatively specifically detect the activity of Glutathione Peroxidase.
Principle
The total glutathione peroxidase activity assay kit (DTNB method) is a colorimetric method for detecting the activity of glutathione peroxidase (GPx). When GSH is relatively sufficient and there is a sufficient amount of organic peroxide, GPx can catalyze GSH to produce GSSG. The remaining GSH can react with the chromogenic substrate DTNB to produce GSSG and yellow TNB. At this time, the level of GPx activity determines the amount of remaining GSH, thereby determining the amount of yellow TNB formed. Therefore, the amount of TNB formed is negatively correlated with the activity of GPx. The activity of glutathione peroxidase can be calculated by measuring the absorbance of yellow TNB.
Since t-Bu-OOH cannot be catalyzed by glutathione peroxidase that does not contain selenium, this kit can be used to quantitatively detect the most common selenium-containing glutathione peroxidase more specifically.
Components
Components | Size(100T) | Storage |
Sample Homogenate | 100ml | -20℃ |
Glutathione Peroxidase Detection Buffer | 50ml | -20℃ |
Reduced Glutathione (GSH) | 4.5mg | -20℃, prepare into solution, store at -20ºC after aliquoting. |
DTNB | 4.5mg | -20℃, prepare into solution, store at -20ºC after aliquoting. |
DMSO | 1.5ml | -20℃ |
Peroxide Reagent(t-Bu-OOH) | 200μl | -20℃ |
Microplate(96 wells) | 1 plate | RT |
Plate Sealer | 2 pieces | RT |
Storage
Unopened kits can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
